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aav carrying cre dependent activator dreadd  (Addgene inc)


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    Addgene inc aav carrying cre dependent activator dreadd
    a-c , Penk -Cre mice were stimulated with 600 mM glucose, and brain slices were analysed for Fos and Penk labelling. Penk neurons were marked by expression of nuclear-localized tdTomato (Ai75D ). b , Low-magnification section of the brainstem (Bregma −7.5 mm) showing Penk expression (red); tissue was counterstained with DAPI (blue), n = 2 independent experiments. Scale bar, 500 μm; cNST, highlighted yellow. c , Sugar-preference neurons are Penk -expressing. Penk neurons labelled with tdTomato (from panel b ) and glucose-activated neurons (Fos-labelled) marked green. Note the high degree of overlap in the merged image. Approximately 85% of sugar-activated cNST neurons are marked by Penk , and ~90% of cNST Penk neurons have sugar-Fos labelling (n = 3 mice). Scale bar, 20 μm. d , The activating <t>DREADD</t> receptor , <t>AAV-DIO-hM3Dq</t> was targeted bilaterally to the cNST of Penk -Cre animals. Mice were then tested for their preference between two flavours for 48 h (PRE). The diagram shows an example using cherry (containing 2 mM AceK) versus grape (with 1 mM AceK). Animals were conditioned and tested using the un-preferred flavour plus the DREADD agonist Clozapine (POST; see ). e , Penk -hM3Dq animals initially prefer the sweeter solution. Remarkably, after associating Clozapine-mediated activation of Penk cNST neurons with the un-preferred flavour, all the Penk -hM3Dq mice significantly switched their preference (PRE = 18.1 ± 2.7 %, POST = 61.1 ± 5.5 %; n = 8 mice, two-sided Mann-Whitney U-Test, p = 1 × 10 −4 ). The experiment was carried out using grape (purple lines) or cherry (red lines) as the initially un-preferred stimuli. f , Mice not expressing the DREADD receptor are unaffected by the presence of Clozapine (PRE = 19.0 ± 3.0 %, POST = 21.4 ± 4.0 %, n = 8 mice); control animals were subjected to the same conditioning and testing as the experimental cohort. Values are mean ± s.e.m.
    Aav Carrying Cre Dependent Activator Dreadd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aav carrying cre dependent activator dreadd/product/Addgene inc
    Average 96 stars, based on 666 article reviews
    aav carrying cre dependent activator dreadd - by Bioz Stars, 2026-05
    96/100 stars

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    1) Product Images from "The Gut-Brain Axis Mediates Sugar Preference"

    Article Title: The Gut-Brain Axis Mediates Sugar Preference

    Journal: Nature

    doi: 10.1038/s41586-020-2199-7

    a-c , Penk -Cre mice were stimulated with 600 mM glucose, and brain slices were analysed for Fos and Penk labelling. Penk neurons were marked by expression of nuclear-localized tdTomato (Ai75D ). b , Low-magnification section of the brainstem (Bregma −7.5 mm) showing Penk expression (red); tissue was counterstained with DAPI (blue), n = 2 independent experiments. Scale bar, 500 μm; cNST, highlighted yellow. c , Sugar-preference neurons are Penk -expressing. Penk neurons labelled with tdTomato (from panel b ) and glucose-activated neurons (Fos-labelled) marked green. Note the high degree of overlap in the merged image. Approximately 85% of sugar-activated cNST neurons are marked by Penk , and ~90% of cNST Penk neurons have sugar-Fos labelling (n = 3 mice). Scale bar, 20 μm. d , The activating DREADD receptor , AAV-DIO-hM3Dq was targeted bilaterally to the cNST of Penk -Cre animals. Mice were then tested for their preference between two flavours for 48 h (PRE). The diagram shows an example using cherry (containing 2 mM AceK) versus grape (with 1 mM AceK). Animals were conditioned and tested using the un-preferred flavour plus the DREADD agonist Clozapine (POST; see ). e , Penk -hM3Dq animals initially prefer the sweeter solution. Remarkably, after associating Clozapine-mediated activation of Penk cNST neurons with the un-preferred flavour, all the Penk -hM3Dq mice significantly switched their preference (PRE = 18.1 ± 2.7 %, POST = 61.1 ± 5.5 %; n = 8 mice, two-sided Mann-Whitney U-Test, p = 1 × 10 −4 ). The experiment was carried out using grape (purple lines) or cherry (red lines) as the initially un-preferred stimuli. f , Mice not expressing the DREADD receptor are unaffected by the presence of Clozapine (PRE = 19.0 ± 3.0 %, POST = 21.4 ± 4.0 %, n = 8 mice); control animals were subjected to the same conditioning and testing as the experimental cohort. Values are mean ± s.e.m.
    Figure Legend Snippet: a-c , Penk -Cre mice were stimulated with 600 mM glucose, and brain slices were analysed for Fos and Penk labelling. Penk neurons were marked by expression of nuclear-localized tdTomato (Ai75D ). b , Low-magnification section of the brainstem (Bregma −7.5 mm) showing Penk expression (red); tissue was counterstained with DAPI (blue), n = 2 independent experiments. Scale bar, 500 μm; cNST, highlighted yellow. c , Sugar-preference neurons are Penk -expressing. Penk neurons labelled with tdTomato (from panel b ) and glucose-activated neurons (Fos-labelled) marked green. Note the high degree of overlap in the merged image. Approximately 85% of sugar-activated cNST neurons are marked by Penk , and ~90% of cNST Penk neurons have sugar-Fos labelling (n = 3 mice). Scale bar, 20 μm. d , The activating DREADD receptor , AAV-DIO-hM3Dq was targeted bilaterally to the cNST of Penk -Cre animals. Mice were then tested for their preference between two flavours for 48 h (PRE). The diagram shows an example using cherry (containing 2 mM AceK) versus grape (with 1 mM AceK). Animals were conditioned and tested using the un-preferred flavour plus the DREADD agonist Clozapine (POST; see ). e , Penk -hM3Dq animals initially prefer the sweeter solution. Remarkably, after associating Clozapine-mediated activation of Penk cNST neurons with the un-preferred flavour, all the Penk -hM3Dq mice significantly switched their preference (PRE = 18.1 ± 2.7 %, POST = 61.1 ± 5.5 %; n = 8 mice, two-sided Mann-Whitney U-Test, p = 1 × 10 −4 ). The experiment was carried out using grape (purple lines) or cherry (red lines) as the initially un-preferred stimuli. f , Mice not expressing the DREADD receptor are unaffected by the presence of Clozapine (PRE = 19.0 ± 3.0 %, POST = 21.4 ± 4.0 %, n = 8 mice); control animals were subjected to the same conditioning and testing as the experimental cohort. Values are mean ± s.e.m.

    Techniques Used: Expressing, Activation Assay, MANN-WHITNEY, Control



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    aav carrying cre dependent activator dreadd  (Addgene inc)
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    Addgene inc aav carrying cre dependent activator dreadd
    a-c , Penk -Cre mice were stimulated with 600 mM glucose, and brain slices were analysed for Fos and Penk labelling. Penk neurons were marked by expression of nuclear-localized tdTomato (Ai75D ). b , Low-magnification section of the brainstem (Bregma −7.5 mm) showing Penk expression (red); tissue was counterstained with DAPI (blue), n = 2 independent experiments. Scale bar, 500 μm; cNST, highlighted yellow. c , Sugar-preference neurons are Penk -expressing. Penk neurons labelled with tdTomato (from panel b ) and glucose-activated neurons (Fos-labelled) marked green. Note the high degree of overlap in the merged image. Approximately 85% of sugar-activated cNST neurons are marked by Penk , and ~90% of cNST Penk neurons have sugar-Fos labelling (n = 3 mice). Scale bar, 20 μm. d , The activating <t>DREADD</t> receptor , <t>AAV-DIO-hM3Dq</t> was targeted bilaterally to the cNST of Penk -Cre animals. Mice were then tested for their preference between two flavours for 48 h (PRE). The diagram shows an example using cherry (containing 2 mM AceK) versus grape (with 1 mM AceK). Animals were conditioned and tested using the un-preferred flavour plus the DREADD agonist Clozapine (POST; see ). e , Penk -hM3Dq animals initially prefer the sweeter solution. Remarkably, after associating Clozapine-mediated activation of Penk cNST neurons with the un-preferred flavour, all the Penk -hM3Dq mice significantly switched their preference (PRE = 18.1 ± 2.7 %, POST = 61.1 ± 5.5 %; n = 8 mice, two-sided Mann-Whitney U-Test, p = 1 × 10 −4 ). The experiment was carried out using grape (purple lines) or cherry (red lines) as the initially un-preferred stimuli. f , Mice not expressing the DREADD receptor are unaffected by the presence of Clozapine (PRE = 19.0 ± 3.0 %, POST = 21.4 ± 4.0 %, n = 8 mice); control animals were subjected to the same conditioning and testing as the experimental cohort. Values are mean ± s.e.m.
    Aav Carrying Cre Dependent Activator Dreadd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aav carrying cre dependent activator dreadd/product/Addgene inc
    Average 96 stars, based on 1 article reviews
    aav carrying cre dependent activator dreadd - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    a-c , Penk -Cre mice were stimulated with 600 mM glucose, and brain slices were analysed for Fos and Penk labelling. Penk neurons were marked by expression of nuclear-localized tdTomato (Ai75D ). b , Low-magnification section of the brainstem (Bregma −7.5 mm) showing Penk expression (red); tissue was counterstained with DAPI (blue), n = 2 independent experiments. Scale bar, 500 μm; cNST, highlighted yellow. c , Sugar-preference neurons are Penk -expressing. Penk neurons labelled with tdTomato (from panel b ) and glucose-activated neurons (Fos-labelled) marked green. Note the high degree of overlap in the merged image. Approximately 85% of sugar-activated cNST neurons are marked by Penk , and ~90% of cNST Penk neurons have sugar-Fos labelling (n = 3 mice). Scale bar, 20 μm. d , The activating DREADD receptor , AAV-DIO-hM3Dq was targeted bilaterally to the cNST of Penk -Cre animals. Mice were then tested for their preference between two flavours for 48 h (PRE). The diagram shows an example using cherry (containing 2 mM AceK) versus grape (with 1 mM AceK). Animals were conditioned and tested using the un-preferred flavour plus the DREADD agonist Clozapine (POST; see ). e , Penk -hM3Dq animals initially prefer the sweeter solution. Remarkably, after associating Clozapine-mediated activation of Penk cNST neurons with the un-preferred flavour, all the Penk -hM3Dq mice significantly switched their preference (PRE = 18.1 ± 2.7 %, POST = 61.1 ± 5.5 %; n = 8 mice, two-sided Mann-Whitney U-Test, p = 1 × 10 −4 ). The experiment was carried out using grape (purple lines) or cherry (red lines) as the initially un-preferred stimuli. f , Mice not expressing the DREADD receptor are unaffected by the presence of Clozapine (PRE = 19.0 ± 3.0 %, POST = 21.4 ± 4.0 %, n = 8 mice); control animals were subjected to the same conditioning and testing as the experimental cohort. Values are mean ± s.e.m.

    Journal: Nature

    Article Title: The Gut-Brain Axis Mediates Sugar Preference

    doi: 10.1038/s41586-020-2199-7

    Figure Lengend Snippet: a-c , Penk -Cre mice were stimulated with 600 mM glucose, and brain slices were analysed for Fos and Penk labelling. Penk neurons were marked by expression of nuclear-localized tdTomato (Ai75D ). b , Low-magnification section of the brainstem (Bregma −7.5 mm) showing Penk expression (red); tissue was counterstained with DAPI (blue), n = 2 independent experiments. Scale bar, 500 μm; cNST, highlighted yellow. c , Sugar-preference neurons are Penk -expressing. Penk neurons labelled with tdTomato (from panel b ) and glucose-activated neurons (Fos-labelled) marked green. Note the high degree of overlap in the merged image. Approximately 85% of sugar-activated cNST neurons are marked by Penk , and ~90% of cNST Penk neurons have sugar-Fos labelling (n = 3 mice). Scale bar, 20 μm. d , The activating DREADD receptor , AAV-DIO-hM3Dq was targeted bilaterally to the cNST of Penk -Cre animals. Mice were then tested for their preference between two flavours for 48 h (PRE). The diagram shows an example using cherry (containing 2 mM AceK) versus grape (with 1 mM AceK). Animals were conditioned and tested using the un-preferred flavour plus the DREADD agonist Clozapine (POST; see ). e , Penk -hM3Dq animals initially prefer the sweeter solution. Remarkably, after associating Clozapine-mediated activation of Penk cNST neurons with the un-preferred flavour, all the Penk -hM3Dq mice significantly switched their preference (PRE = 18.1 ± 2.7 %, POST = 61.1 ± 5.5 %; n = 8 mice, two-sided Mann-Whitney U-Test, p = 1 × 10 −4 ). The experiment was carried out using grape (purple lines) or cherry (red lines) as the initially un-preferred stimuli. f , Mice not expressing the DREADD receptor are unaffected by the presence of Clozapine (PRE = 19.0 ± 3.0 %, POST = 21.4 ± 4.0 %, n = 8 mice); control animals were subjected to the same conditioning and testing as the experimental cohort. Values are mean ± s.e.m.

    Article Snippet: For gain of preference experiments, Penk -Cre animals were stereotaxically injected with 300 nl of AAV carrying Cre-dependent activator DREADD (1–2 × 10 13 GC/ml; AAV9 Syn-DIO-hM3Dq-mCherry, Addgene #44361), bilaterally in the cNST.

    Techniques: Expressing, Activation Assay, MANN-WHITNEY, Control